Another run with 12 samples was performed on the 32-well block during which a temperature of 99°C was maintained on the 64-well block. Results and discussion (1) PCR optimization via gradient function ...

Producing high yields of good-quality PCR products requires a complex combination of optimal chemistry, primer design, template quality, and cycling parameters. The details of these reaction ...

Promega Corporation introduces Promega PCR Optimization Kit that allows customers to rapidly define their own unique PCR master mix manufactured under cGMP for research or clinical use. The user’s ...

Common problems that arise during PCR assays include weak target-binding, competing target secondary structure, primer-dimer formation, and mishybridization of PCR probes and primers. These issues, ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

SuperScript IV UniPrime One-Step RT-PCR System is designed to perform both cDNA synthesis and subsequent PCR amplification in a single tube. This master mix formulation combines the high processivity ...