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3:55
YouTube
Shomu's Biology
rtPCR animation
RT PCR animation - This lecture explains about the RT PCR also known as the real time PCR. Realtime PCR is a technique of amplifying DNA fragments with polymerase chain reaction and along with the PCR. it can help us to monitor the concentration of amplified DNA Replication in real time with the help of fluorescence emission. Real time PCR or ...
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Jun 30, 2016
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Arrange the steps of diagnosing AIDS using the RT PCR method in the right order.I. DNA, engineered primers specific to HIV genes, and nucleotides along with buffers are incubated in a thermal cycler.II. Viral RNA is extracted from the patient’s plasma or blood sample.III. The PCR product is run on a gel to separate and identify the PCR amplified viral genes.IV. Reverse transcriptase is used to convert the RNA to cDNA.
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